diff --git a/rest_framework/relations.py b/rest_framework/relations.py
index 744823c48..0bcdcdd95 100644
--- a/rest_framework/relations.py
+++ b/rest_framework/relations.py
@@ -69,10 +69,6 @@ class RelatedField(Field):
             'Relational field must provide a `queryset` argument, '
             'or set read_only=`True`.'
         )
-        assert not (self.queryset is not None and kwargs.get('read_only', None)), (
-            'Relational fields should not provide a `queryset` argument, '
-            'when setting read_only=`True`.'
-        )
         kwargs.pop('many', None)
         kwargs.pop('allow_empty', None)
         super(RelatedField, self).__init__(**kwargs)