diff --git a/src/dependency_injector/wiring.py b/src/dependency_injector/wiring.py
index 37aba496..3f998393 100644
--- a/src/dependency_injector/wiring.py
+++ b/src/dependency_injector/wiring.py
@@ -321,7 +321,7 @@ class InspectFilter:
     def _is_starlette_request_cls(self, instance: object) -> bool:
         return starlette \
                and isinstance(instance, type) \
-               and issubclass(instance, starlette.requests.Request)
+               and isinstance(instance, starlette.requests.Request)
 
     def _is_builtin(self, instance: object) -> bool:
         return inspect.isbuiltin(instance)