diff --git a/src/PIL/ImageQt.py b/src/PIL/ImageQt.py
index e142f1f27..f10de3258 100644
--- a/src/PIL/ImageQt.py
+++ b/src/PIL/ImageQt.py
@@ -108,7 +108,7 @@ def align8to32(bytes, width, mode):
     converts each scanline of data from 8 bit to 32 bit aligned
     """
 
-    bits_per_pixel = {"1": 1, "L": 8, "P": 8}[mode]
+    bits_per_pixel = {"1": 1, "L": 8, "P": 8, "I;16": 16}[mode]
 
     # calculate bytes per line and the extra padding if needed
     bits_per_line = bits_per_pixel * width
@@ -167,6 +167,8 @@ def _toqclass_helper(im):
     elif im.mode == "RGBA":
         data = im.tobytes("raw", "BGRA")
         format = qt_format.Format_ARGB32
+    elif im.mode == "I;16":
+        format = qt_format.Format_Grayscale16
     else:
         if exclusive_fp:
             im.close()