diff --git a/PIL/ImageFont.py b/PIL/ImageFont.py
index 9366937dd..18d09b871 100644
--- a/PIL/ImageFont.py
+++ b/PIL/ImageFont.py
@@ -396,7 +396,7 @@ w7IkEbzhVQAAAABJRU5ErkJggg==
 if __name__ == "__main__":
     # create font data chunk for embedding
     import base64, os, sys
-    font = "../Images/courB08"
+    font = "../Tests/images/courB08"
     print("    f._load_pilfont_data(")
     print("         # %s" % os.path.basename(font))
     print("         BytesIO(base64.decodestring(b'''")
diff --git a/Tests/helper.py b/Tests/helper.py
index c203e67c2..aacbfc009 100644
--- a/Tests/helper.py
+++ b/Tests/helper.py
@@ -233,7 +233,7 @@ def lena(mode="RGB", cache={}):
     # im = cache.get(mode)
     if im is None:
         if mode == "RGB":
-            im = Image.open("Images/lena.ppm")
+            im = Image.open("Tests/images/lena.ppm")
         elif mode == "F":
             im = lena("L").convert(mode)
         elif mode[:4] == "I;16":
diff --git a/Tests/test_file_fli.py b/Tests/test_file_fli.py
index 787365c14..a98a80b78 100644
--- a/Tests/test_file_fli.py
+++ b/Tests/test_file_fli.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 from PIL import Image
 
 # sample ppm stream
-file = "Images/lena.fli"
+file = "Tests/images/lena.fli"
 data = open(file, "rb").read()
 
 
diff --git a/Tests/test_file_gif.py b/Tests/test_file_gif.py
index 3aefbe9b3..01ac1d95c 100644
--- a/Tests/test_file_gif.py
+++ b/Tests/test_file_gif.py
@@ -5,7 +5,7 @@ from PIL import Image
 codecs = dir(Image.core)
 
 # sample gif stream
-file = "Images/lena.gif"
+file = "Tests/images/lena.gif"
 with open(file, "rb") as f:
     data = f.read()
 
@@ -45,7 +45,7 @@ class TestFileGif(PillowTestCase):
     def test_roundtrip2(self):
         # see https://github.com/python-pillow/Pillow/issues/403
         out = self.tempfile('temp.gif')
-        im = Image.open('Images/lena.gif')
+        im = Image.open('Tests/images/lena.gif')
         im2 = im.copy()
         im2.save(out)
         reread = Image.open(out)
@@ -55,7 +55,7 @@ class TestFileGif(PillowTestCase):
     def test_palette_handling(self):
         # see https://github.com/python-pillow/Pillow/issues/513
 
-        im = Image.open('Images/lena.gif')
+        im = Image.open('Tests/images/lena.gif')
         im = im.convert('RGB')
 
         im = im.resize((100, 100), Image.ANTIALIAS)
diff --git a/Tests/test_file_icns.py b/Tests/test_file_icns.py
index c307ec844..f19eb16b7 100644
--- a/Tests/test_file_icns.py
+++ b/Tests/test_file_icns.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 from PIL import Image
 
 # sample icon file
-file = "Images/pillow.icns"
+file = "Tests/images/pillow.icns"
 data = open(file, "rb").read()
 
 enable_jpeg2k = hasattr(Image.core, 'jp2klib_version')
diff --git a/Tests/test_file_ico.py b/Tests/test_file_ico.py
index 5bad94a53..165d10225 100644
--- a/Tests/test_file_ico.py
+++ b/Tests/test_file_ico.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 from PIL import Image
 
 # sample ppm stream
-file = "Images/lena.ico"
+file = "Tests/images/lena.ico"
 data = open(file, "rb").read()
 
 
diff --git a/Tests/test_file_jpeg.py b/Tests/test_file_jpeg.py
index c21910962..158299c36 100644
--- a/Tests/test_file_jpeg.py
+++ b/Tests/test_file_jpeg.py
@@ -8,7 +8,7 @@ from PIL import ImageFile
 
 codecs = dir(Image.core)
 
-test_file = "Images/lena.jpg"
+test_file = "Tests/images/lena.jpg"
 
 
 class TestFileJpeg(PillowTestCase):
@@ -215,7 +215,7 @@ class TestFileJpeg(PillowTestCase):
         self.assertEqual(info[305], 'Adobe Photoshop CS Macintosh')
 
     def test_quality_keep(self):
-        im = Image.open("Images/lena.jpg")
+        im = Image.open("Tests/images/lena.jpg")
         f = self.tempfile('temp.jpg')
         im.save(f, quality='keep')
 
diff --git a/Tests/test_file_png.py b/Tests/test_file_png.py
index 6da61be6b..145eff327 100644
--- a/Tests/test_file_png.py
+++ b/Tests/test_file_png.py
@@ -10,7 +10,7 @@ codecs = dir(Image.core)
 
 # sample png stream
 
-file = "Images/lena.png"
+file = "Tests/images/lena.png"
 data = open(file, "rb").read()
 
 # stuff to create inline PNG images
@@ -204,10 +204,10 @@ class TestFilePng(PillowTestCase):
     def test_load_verify(self):
         # Check open/load/verify exception (@PIL150)
 
-        im = Image.open("Images/lena.png")
+        im = Image.open("Tests/images/lena.png")
         im.verify()
 
-        im = Image.open("Images/lena.png")
+        im = Image.open("Tests/images/lena.png")
         im.load()
         self.assertRaises(RuntimeError, lambda: im.verify())
 
diff --git a/Tests/test_file_ppm.py b/Tests/test_file_ppm.py
index 9d5dd806a..d9e4e0674 100644
--- a/Tests/test_file_ppm.py
+++ b/Tests/test_file_ppm.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 from PIL import Image
 
 # sample ppm stream
-file = "Images/lena.ppm"
+file = "Tests/images/lena.ppm"
 data = open(file, "rb").read()
 
 
diff --git a/Tests/test_file_psd.py b/Tests/test_file_psd.py
index e7f86fd98..007646901 100644
--- a/Tests/test_file_psd.py
+++ b/Tests/test_file_psd.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 from PIL import Image
 
 # sample ppm stream
-file = "Images/lena.psd"
+file = "Tests/images/lena.psd"
 data = open(file, "rb").read()
 
 
diff --git a/Tests/test_file_tar.py b/Tests/test_file_tar.py
index 36a625add..7e36f35fc 100644
--- a/Tests/test_file_tar.py
+++ b/Tests/test_file_tar.py
@@ -5,7 +5,7 @@ from PIL import Image, TarIO
 codecs = dir(Image.core)
 
 # sample ppm stream
-tarfile = "Images/lena.tar"
+tarfile = "Tests/images/lena.tar"
 
 
 class TestFileTar(PillowTestCase):
diff --git a/Tests/test_file_webp.py b/Tests/test_file_webp.py
index ea1c2e19f..1eeea57d3 100644
--- a/Tests/test_file_webp.py
+++ b/Tests/test_file_webp.py
@@ -23,7 +23,7 @@ class TestFileWebp(PillowTestCase):
 
     def test_read_rgb(self):
 
-        file_path = "Images/lena.webp"
+        file_path = "Tests/images/lena.webp"
         image = Image.open(file_path)
 
         self.assertEqual(image.mode, "RGB")
@@ -33,7 +33,7 @@ class TestFileWebp(PillowTestCase):
         image.getdata()
 
         # generated with:
-        # dwebp -ppm ../../Images/lena.webp -o lena_webp_bits.ppm
+        # dwebp -ppm ../../Tests/images/lena.webp -o lena_webp_bits.ppm
         target = Image.open('Tests/images/lena_webp_bits.ppm')
         self.assert_image_similar(image, target, 20.0)
 
diff --git a/Tests/test_file_webp_alpha.py b/Tests/test_file_webp_alpha.py
index c9787c60e..0df3143bb 100644
--- a/Tests/test_file_webp_alpha.py
+++ b/Tests/test_file_webp_alpha.py
@@ -22,7 +22,7 @@ class TestFileWebpAlpha(PillowTestCase):
 
     def test_read_rgba(self):
         # Generated with `cwebp transparent.png -o transparent.webp`
-        file_path = "Images/transparent.webp"
+        file_path = "Tests/images/transparent.webp"
         image = Image.open(file_path)
 
         self.assertEqual(image.mode, "RGBA")
@@ -33,7 +33,7 @@ class TestFileWebpAlpha(PillowTestCase):
 
         image.tobytes()
 
-        target = Image.open('Images/transparent.png')
+        target = Image.open('Tests/images/transparent.png')
         self.assert_image_similar(image, target, 20.0)
 
     def test_write_lossless_rgb(self):
diff --git a/Tests/test_file_webp_metadata.py b/Tests/test_file_webp_metadata.py
index 93021ac07..2470f4c49 100644
--- a/Tests/test_file_webp_metadata.py
+++ b/Tests/test_file_webp_metadata.py
@@ -15,7 +15,7 @@ class TestFileWebpMetadata(PillowTestCase):
 
     def test_read_exif_metadata(self):
 
-        file_path = "Images/flower.webp"
+        file_path = "Tests/images/flower.webp"
         image = Image.open(file_path)
 
         self.assertEqual(image.format, "WEBP")
@@ -54,7 +54,7 @@ class TestFileWebpMetadata(PillowTestCase):
 
     def test_read_icc_profile(self):
 
-        file_path = "Images/flower2.webp"
+        file_path = "Tests/images/flower2.webp"
         image = Image.open(file_path)
 
         self.assertEqual(image.format, "WEBP")
diff --git a/Tests/test_file_xpm.py b/Tests/test_file_xpm.py
index 0c765da8e..e6e750298 100644
--- a/Tests/test_file_xpm.py
+++ b/Tests/test_file_xpm.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 from PIL import Image
 
 # sample ppm stream
-file = "Images/lena.xpm"
+file = "Tests/images/lena.xpm"
 data = open(file, "rb").read()
 
 
diff --git a/Tests/test_font_bdf.py b/Tests/test_font_bdf.py
index 9a8f19d03..ce5a371e0 100644
--- a/Tests/test_font_bdf.py
+++ b/Tests/test_font_bdf.py
@@ -2,7 +2,7 @@ from helper import unittest, PillowTestCase, tearDownModule
 
 from PIL import FontFile, BdfFontFile
 
-filename = "Images/courB08.bdf"
+filename = "Tests/images/courB08.bdf"
 
 
 class TestFontBdf(PillowTestCase):
diff --git a/Tests/test_image_convert.py b/Tests/test_image_convert.py
index 946dddc11..1415bae3a 100644
--- a/Tests/test_image_convert.py
+++ b/Tests/test_image_convert.py
@@ -36,7 +36,7 @@ class TestImageConvert(PillowTestCase):
         self.assertEqual(orig, converted)
 
     def test_8bit(self):
-        im = Image.open('Images/lena.jpg')
+        im = Image.open('Tests/images/lena.jpg')
         self._test_float_conversion(im.convert('L'))
 
     def test_16bit(self):
diff --git a/Tests/test_image_draft.py b/Tests/test_image_draft.py
index e970c9794..252e60376 100644
--- a/Tests/test_image_draft.py
+++ b/Tests/test_image_draft.py
@@ -3,7 +3,7 @@ from helper import unittest, PillowTestCase, tearDownModule, fromstring, tostrin
 from PIL import Image
 
 codecs = dir(Image.core)
-filename = "Images/lena.jpg"
+filename = "Tests/images/lena.jpg"
 data = tostring(Image.open(filename).resize((512, 512)), "JPEG")
 
 
diff --git a/Tests/test_image_load.py b/Tests/test_image_load.py
index cf23a0101..14cb76fdb 100644
--- a/Tests/test_image_load.py
+++ b/Tests/test_image_load.py
@@ -16,14 +16,14 @@ class TestImageLoad(PillowTestCase):
         self.assertEqual(pix[0, 0], (223, 162, 133))
 
     def test_close(self):
-        im = Image.open("Images/lena.gif")
+        im = Image.open("Tests/images/lena.gif")
         im.close()
         self.assertRaises(ValueError, lambda: im.load())
         self.assertRaises(ValueError, lambda: im.getpixel((0, 0)))
 
     def test_contextmanager(self):
         fn = None
-        with Image.open("Images/lena.gif") as im:
+        with Image.open("Tests/images/lena.gif") as im:
             fn = im.fp.fileno()
             os.fstat(fn)
 
diff --git a/Tests/test_imageops_usm.py b/Tests/test_imageops_usm.py
index 2f81eebce..486b201ab 100644
--- a/Tests/test_imageops_usm.py
+++ b/Tests/test_imageops_usm.py
@@ -4,7 +4,7 @@ from PIL import Image
 from PIL import ImageOps
 from PIL import ImageFilter
 
-im = Image.open("Images/lena.ppm")
+im = Image.open("Tests/images/lena.ppm")
 
 
 class TestImageOpsUsm(PillowTestCase):
diff --git a/Tests/test_locale.py b/Tests/test_locale.py
index 7ef63634b..0465fb207 100644
--- a/Tests/test_locale.py
+++ b/Tests/test_locale.py
@@ -19,7 +19,7 @@ import locale
 
 # one of string.whitespace is not freely convertable into ascii.
 
-path = "Images/lena.jpg"
+path = "Tests/images/lena.jpg"
 
 
 class TestLocale(PillowTestCase):
diff --git a/Tests/test_pickle.py b/Tests/test_pickle.py
index 5f3ae6f51..304baf964 100644
--- a/Tests/test_pickle.py
+++ b/Tests/test_pickle.py
@@ -6,7 +6,7 @@ from PIL import Image
 class TestPickle(PillowTestCase):
 
     def helper_pickle_file(self, pickle, protocol=0):
-        im = Image.open('Images/lena.jpg')
+        im = Image.open('Tests/images/lena.jpg')
         filename = self.tempfile('temp.pkl')
 
         # Act
@@ -19,7 +19,7 @@ class TestPickle(PillowTestCase):
         self.assertEqual(im, loaded_im)
 
     def helper_pickle_string(
-            self, pickle, protocol=0, file='Images/lena.jpg'):
+            self, pickle, protocol=0, file='Tests/images/lena.jpg'):
         im = Image.open(file)
 
         # Act
diff --git a/Tests/tester.py b/Tests/tester.py
index 4476aced1..866009251 100644
--- a/Tests/tester.py
+++ b/Tests/tester.py
@@ -215,7 +215,7 @@ def lena(mode="RGB", cache={}):
     im = cache.get(mode)
     if im is None:
         if mode == "RGB":
-            im = Image.open("Images/lena.ppm")
+            im = Image.open("Tests/images/lena.ppm")
         elif mode == "F":
             im = lena("L").convert(mode)
         elif mode[:4] == "I;16":
diff --git a/Tests/threaded_save.py b/Tests/threaded_save.py
index 471e24815..12fcff2cf 100644
--- a/Tests/threaded_save.py
+++ b/Tests/threaded_save.py
@@ -9,7 +9,7 @@ try:
 except:
     format = "PNG"
 
-im = Image.open("Images/lena.ppm")
+im = Image.open("Tests/images/lena.ppm")
 im.load()
 
 queue = queue.Queue()
diff --git a/selftest.py b/selftest.py
index 248cb3937..29af34ad2 100644
--- a/selftest.py
+++ b/selftest.py
@@ -49,13 +49,13 @@ def testimage():
 
     Or open existing files:
 
-    >>> im = Image.open(os.path.join(ROOT, "Images/lena.gif"))
+    >>> im = Image.open(os.path.join(ROOT, "Tests/images/lena.gif"))
     >>> _info(im)
     ('GIF', 'P', (128, 128))
-    >>> _info(Image.open(os.path.join(ROOT, "Images/lena.ppm")))
+    >>> _info(Image.open(os.path.join(ROOT, "Tests/images/lena.ppm")))
     ('PPM', 'RGB', (128, 128))
     >>> try:
-    ...  _info(Image.open(os.path.join(ROOT, "Images/lena.jpg")))
+    ...  _info(Image.open(os.path.join(ROOT, "Tests/images/lena.jpg")))
     ... except IOError as v:
     ...  print(v)
     ('JPEG', 'RGB', (128, 128))
@@ -63,7 +63,7 @@ def testimage():
     PIL doesn't actually load the image data until it's needed,
     or you call the "load" method:
 
-    >>> im = Image.open(os.path.join(ROOT, "Images/lena.ppm"))
+    >>> im = Image.open(os.path.join(ROOT, "Tests/images/lena.ppm"))
     >>> print(im.im) # internal image attribute
     None
     >>> a = im.load()
@@ -73,7 +73,7 @@ def testimage():
     You can apply many different operations on images.  Most
     operations return a new image:
 
-    >>> im = Image.open(os.path.join(ROOT, "Images/lena.ppm"))
+    >>> im = Image.open(os.path.join(ROOT, "Tests/images/lena.ppm"))
     >>> _info(im.convert("L"))
     (None, 'L', (128, 128))
     >>> _info(im.copy())